Advances in CRISPR/Cas9 gene-editing system
10.3969/j.issn.1001-6325.2018.04.025
- VernacularTitle:CRISPR/Cas9基因编辑系统研究进展
- Author:
Cheng CHENG
1
;
Peng-Cheng SHU
;
Xiao-Zhong PENG
Author Information
1. 中国医学科学院 北京协和医学院
- Keywords:
CRISPR/Cas9;
gene editing;
off-target effect;
editing efficiency
- From:
Basic & Clinical Medicine
2018;38(4):543-547
- CountryChina
- Language:Chinese
-
Abstract:
CRISPR/Cas9 gene-editing system has been broadly used in various fields of bioscience and medicine in recent years. The system can be guided by RNA to specific DNA site thus achieving targeted gene editing.Off-target effect and editing efficiency remain to be two crucial challeges to the system. Currently, a number of researches have been focused on the optimization of the system by reducing off-target effects and increasing editing efficiency, which may enhance its safety and expand its application.
