Isolation and culture of adult rat cardiac microvascular endothelial cells
10.3969/j.issn.1001-6325.2018.04.021
- VernacularTitle:成年大鼠心肌微血管内皮细胞的分离与培养
- Author:
Xiao-Yan CHEN
1
;
Meng-Ya FAN
;
Hui LI
;
Yi-Nan JIANG
;
Jing JING
;
Hai-Long ZHANG
Author Information
1. 河南大学 医学院 抗体药物开发技术国家地方联合工程实验室 河南省抗体药物国际联合实验室细胞与分子免疫重点实验室
- Keywords:
cell culture;
endothelial cells;
adult rat;
cardiac microvascular
- From:
Basic & Clinical Medicine
2018;38(4):530-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and efficient method for isolation and culture of adult rat cardiac micro-vascular endothelial cells. Methods Wistar rats were anesthetized and thoracotomy was performed. Cardiac micro-vascular endothelial cells were isolated by heart perfusion and enzyme digestion. The morphology and growth curve of the third generation cells were examined by inverted microscopy. Its molecular markers factor Ⅷ-related antigen and CD31 were detected by immunocytochemistry and immunofluoresence. The angiogenic ability was examined by matrigel. Results The primary cultured cardiac microvascular endothelial cells in vitro were polygonal or fusiform with typical paving stone-like growth characteristics. At the same time,the cells were positive stained by factorⅧ-related antigen and CD31 and showed angiogenic ability. Conclusions Cardiac microvascular endothelial cells cul-tured by this method possess high purity. The method is easy to operate and can be used for laboratory and clinical research of cardiovascular diseases.
