Effects of hypoxia on the expression of autophagy-related molecules in human colon cancer cells
10.3969/j.issn.1001-6325.2018.04.019
- VernacularTitle:低氧对人结肠癌细胞自噬相关分子表达的影响
- Author:
Ya-Ting LIU
1
;
Xin LI
;
Na WANG
;
Fei-Xue SONG
Author Information
1. 兰州大学第二医院 肿瘤内科
- Keywords:
hypoxia;
autophagy;
HIF;
LC3;
CD133+
- From:
Basic & Clinical Medicine
2018;38(4):522-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of hypoxia on SW480 cell autophagy.Methods We screened the CD133+cell with the characteristics of stem cell by magnetic cell separation. Then SW480 cell and CD133+cell were incubated in hypoxic conditions. Subsequently,we detected HIF-1α expression by immunofluorescence(IF), LC3 expression by Western blot and HIF-2α and Beclin 1 expression in 94 colon cancer tissue samples by immuno-histochemistry. Results Hypoxia induced the activation of HIF-1α in SW480 cell. After hypoxia 24 h, LC3 ex-pression was increased in both SW480 cell and CD133+cell with incubation time. The conversion from LC3Ⅰto LC3Ⅱwas increased in CD133+cell,but decreased in SW480 cell. In olon cancer tissue samples,HIF-2α and Be-clin 1 expression in poorly differentiated group were higher than that of well-differentiated group.HIF-2α expression in non-lymph node metastasis group was higher than high lymphatic metastasis group. However the Beclin expression was in the opposite regulation. Conclusions Hypoxia can induce the SW480 apoptosis and a few of CD133+cell autophagy that can protect the cell from apoptosis by inducing the HIF expression and increasing LC3.
