bFGF promotes blood spinal cord barrier recovery in rats
10.3969/j.issn.1001-6325.2018.04.015
- VernacularTitle:bFGF促进大鼠血脊髓屏障的修复
- Author:
Xie ZHANG
1
;
Hua ZHOU
;
Hong-Yu ZHANG
;
Jian XIAO
Author Information
1. 宁波市医疗中心李惠利医院 药剂科
- Keywords:
bFGF;
spinal cord injury;
adhesive connection protein;
tight junction protein expression;
blood spinal cord barrier
- From:
Basic & Clinical Medicine
2018;38(4):502-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of basic fibroblast growth factor(bFGF) promoting blood spi-nal cord barrier(BSCB) recovery in rats after spinal cord injury(SCI).Methods Rats were randomly divided into sham group,SCI model group, bFGF intervention group(80 μg/kg). The nerve function of hind limb motor was evaluated by Basso, Beattie, and Bresnahan (BBB) scores during postoperative 14 days. Neuron loss of injured spinal cords was observed by haematoxylin and eosin(HE) staining and NeuN staining.The integrity of BSCB was investigated with Evan's Blue staining and fluorescein isothiocyanate (FITC)-dextran extravasation. The expression protein of adhesive connection protein (p120-catenin,β-catenin) and tight junction protein expression(occludin, claudin-5) were analyzed by Western blot.Results Compared with SCI model group, bFGF intervention group neuron loss decreased significantly at 3 d after injury(P<0.05);the permeability of blood spinal cord barrier obvi-ously reduced at 1d after injury; the expressions of p120-catenin, beta-catenin, occludin, claudin-5 protein of bFGF intervention group were increased dramatically at 1 d after injury(P<0.05). Conclusions bFGF improves the recovery of BSCB in an SCI model by reducing the loss of neurons and increasing the expressions of adhesion junction proteins and tight juction proteins.
