Effects of ursolic acid on activation of NOX2/ROS/NLRP3 inflammasome in liver fibrosis rats
10.3969/j.issn.1001-6325.2018.04.012
- VernacularTitle:熊果酸对肝纤维化大鼠NOX2/ROS/NLRP3炎性小体活化的影响
- Author:
Chen-Kai HUANG
1
;
Da-Kai GAN
;
Wang ZHANG
;
Fang-Yun LUO
;
Jiang CHEN
;
An-Jiang WANG
;
Bi-Min LI
;
Xuan ZHU
Author Information
1. 南昌大学第一附属医院 消化内科
- Keywords:
ursolic acid;
carbon tetrachloride;
liver fibrosis;
NADPH oxidase 2;
NLRP3 inflammasome
- From:
Basic & Clinical Medicine
2018;38(4):485-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ursolic acid(UA) on NOX2/ROS/NLRP3 inflammasome activation in carbon tetrachloride(CCl4)-induced liver fibrosis SD rat,and to observe the improvement of collagen deposition in liver tissues. Methods All rats were randomly divided into 3 group:control group,CCl4model group,UA treatment group. Liver fibrosis model SD rats was established by the CCl4-induced method and half of them was used as UA treatment group. Serum ALT was detected by ALT detection kit.The liver pathology and collagen deposition were ob-served by HE and Sirius-red staining. The mRNA expression of Nox2,Nlrp3,Caspase1,IL-1β in liver tissues was detected by RT-qPCR. The protein expression of NOX2,NLRP3,caspase-1 and IL-1β in liver tissues was detected by Western blot and immunohistochemistry and the ROS generation in liver tissues was detected by DCFH-DA fluores-cence probe. Results Compared with control group,in the CCl4model group,the serum ALT was much higher (P<0.05);the Ishak's fibrosis score and collagen deposition was significantly increased(P<0.05) and mRNA of Nox2, Nlrp3,Caspase1,IL-1β was increased.In addition,both the NOX2,NLRP3,caspase-1 p10 and IL-1β protein expres-sion and ROS level (P<0.05) of CCl4model group were significant increased.Compared with CCl4model group,in the UA treatment group Ishak's fibrosis score,collagen deposition and ALT decreased.Both mRNA expression of the Nox2, Nlrp3,Caspase1,IL-1β and protein expression of NOX2,NLRP3,caspase-1 p10 and IL-1β as well as ROS were signif-icant decreased,but the caspase-1 p45 protein level has no difference among all these groups (P>0.05). Conclusions Ursolic acid attenuates the liver injury and reduces the collagen deposition,which may relate to its inhibitory effects on NOX2/ROS/NLRP3 inflammasome activation to reduce IL-1β releasing.
