siRNA-mediated antizyme inhibitor down-regulates the expression of ornithine decarboxylase of prostate cancer cell PC3
10.3969/j.issn.1001-6325.2018.04.010
- VernacularTitle:siRNA靶向抗酶抑制剂下调前列腺癌细胞PC3中鸟氨酸脱羧酶表达
- Author:
Tao JIN
1
;
Qian LI
;
Jie OU
;
Yan-Lin WANG
;
Hong-Yan WU
Author Information
1. 三峡大学人民医院 (宜昌市第一人民医院)
- Keywords:
antizyme inhibitor;
ornithine decarboxylase;
antizyme;
siRNA
- From:
Basic & Clinical Medicine
2018;38(4):475-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate whether down-regulating antizyme inhibitor(AZIN) can regulate the expression of ornithine decarboxylase(ODC) and the proliferation of prostate cancer cell PC3 or not.Methods siRNA-AZIN transfected prostate cancer cell PC3,the level of antizyme(AZ),AZIN and ODC were measured by RT-PCR and westernblot. MTT was used to measure the proliferation of cells. Results The mRNA level of AZIN declined(P<0.01);the protein level of AZIN and ODC declined(P<0.05). Knockdown of AZIN significantly inhibited the proliferation of PC3(P<0.05). Conclusions Transfecting siRNA-AZIN can decrease the level of AZIN, then the decline level of ODC inhibits the proliferation of PC3.
