Up-regulation of GABAAα1in ventrolateral periaqueductal gray in rats is associated with formalin-induced acute pain
- VernacularTitle:大鼠中脑导水管周围灰质腹外侧区GABAAα1表达上调与甲醛致急性疼痛有关
- Author:
Wan-Qiu SUN
1
;
Gui-Zhi WANG
;
Chao LOU
;
Chun-Yan ZHANG
;
Yu-Ping JI
;
Jun LI
;
Jian-Feng YU
Author Information
1. 潍坊医学院 麻醉学系
- Keywords:
acute pain;
ventrolateral periaqueductal gray,vLPAG;
GABAAα1
- From:
Basic & Clinical Medicine
2018;38(3):381-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of γ-aminobutyric acid A receptor α1 subunit (GABAAα1) in the ventrolateral periaqueductal gray (vLPAG) in rats with formalin-induced acute pain. Methods The rats were randomly divided into two groups:control group(group C) and formalin-induced pain group(group F),12 rats in each group:0.9% sodium chloride solution or 2% formaldehyde 50 μL was injected into the ventral surface of right hind paw respectively. The pain scores were recorded for every 5 minutes and the mechanical pain threshold were recorded for every 10 minutes until 1 h. The expression levels of GABAAα1in vLPAG were determined by Western blot analysis in each group.Results The rats in formalin group showed significant nociceptive behaviors immedi-ately, such as paw withdrawal and/or paw licking. Results demonstrated that the rats exhibited a biphasic response to pain. The pain behavior scores in group F were significantly higher than that in group C (P<0.05),and the mechanical pain threshold in group F was decreased after injection compared with group C(P<0.05). The expression of GABAAα1 protein in group F was significantly higher than that in group C (P<0.05).Conclusions The up-regulation of GABAAα1 expression in ventrolateral periaqueductal gray is associated with the decrease of pain threshold in rats with acute pain.
