Orexin-B inhibits cerebral ischemia reperfusion injury in rats
- VernacularTitle:食欲素-B抑制大鼠脑缺血再灌注损伤
- Author:
Chao XU
1
;
Chun-Mei WANG
;
Bo BAI
Author Information
1. 山东大学 齐鲁医学部
- Keywords:
orexin-B;
brain ischemia-reperfusion;
phosphorylation of protein kinase b;
glycogen synthase kinase 3β
- From:
Basic & Clinical Medicine
2018;38(3):340-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the neuroprotective effect of orexin-B on rat model of cerebral ischemia-reperfusion injury and its molecular mechanism. Methods The artery occlusion model of male Wister rats(middle cerebral ar-tery occlusion,MCAO) was established which has been ischemic 2 h and reperfusion 24 h. Rats were randomly di-vided into sham group (control), ischemia-reperfusion group (I/R), ischemia-reperfusion +PBS group (I/R+PBS),and ischemia-reperfusion +orexin-B group (I/R+OXB). The neurological deficit scores were processed to inclusion and exclusion. Infarct size was determined by TTC staining;Using Western blot,the expressions of orexin receptor 2,p-AKT,p-GSK-3β proteins in hippocampus were detected;Jumping test was used to detect learning and memory abilities in rats. Results Orexin-B significantly reduced the volume of cerebral infarction in TTC staining;orexin-B group was significantly increased the expression of orexin receptor 2as well as p-AKT,which decreased p-GSK-3β (P<0.05),compared with the untreated group. Furthmore,the orexin-B treated group can improve the latency period and decline the mistakes in rat Jumping test(P<0.05). Conclusions The neuroprotective effect of orexin-B in cerebral ischemia-reperfusion injury may enhance p-AKT activity and inhibit p-GSK-3β activity,which may increase the proliferation of neurons and improve the cerebral blood glucose concentration.
