Preparation of antibody against mouse UPF1 and the expression of UPF1 in adipose cell differentiation
- VernacularTitle:抗小鼠移码因子1蛋白多抗制备及其在脂肪细胞分化中的表达
- Author:
Jun-Yi WANG
1
;
Jian-Qiang ZHU
;
De-Zheng JIA
;
Xin YUAN
;
Paziliye YASEN
;
Ya-Qun GUAN
;
Xiao-Di LIANG
Author Information
1. 新疆医科大学 基础医学院 生物化学与分子生物学教研室
- Keywords:
adipogenesis;
UPF1;
nonsense mediated mRNA decad(NMD);
antibody preparation
- From:
Basic & Clinical Medicine
2018;38(3):335-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare polyclonal antibodies against mouse UPF1 protein and to investigate the expression of UPF1 protein during adipocyte differentiation. Methods UPF1 protein expression vector was constructed to prepare and purify rabbit UPF1 antibody. The differentation of 3T3-L1 cells was induced and the expression of UPF1 was detected by CoIP. Results 1)High specific mUPF1 polyclonal antibody was prepared and the titer of this anti-body reached 640 000;2)The expression of UPF1 protein did not change during adipogenesis;3)In the process of adipocyte differentiation,interaction of UPF1 and UPF2 was increased. Conclusions 1)The polyclonal antibodies prepared by using 550 amino acids at the C terminal of mUPF1 protein could effectively recognize intact mUPF1 pro-tein;2)The interaction of UPF1 protein with UPF2 protein during adipocyte differentiation is enhanced.
