Effects of miR-143 target-regulating ER-α36 on the invasion of gastric cancer cell line SGC 7901
- VernacularTitle:miR-143靶向调控ER-α36影响胃癌细胞系SGC7901的侵袭
- Author:
Feng ZOU
1
;
Yuan-Yuan LUO
;
Li-Jiang LIU
Author Information
1. 江汉大学 医学院病理学与病理生理学教研室
- Keywords:
gastric cancer;
ER-α36;
cell invasion;
miR-143
- From:
Basic & Clinical Medicine
2018;38(3):330-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ER-α36 on invasion of human gastric cancer cell lines SGC7901 by miR-143. Methods Lentiviral vectors were constructed to generate up-and down-regulations of miR-143 lentiviruses (LV-miR-143 and LV-anti-miR-143,respectively).The viruses were used to infect human gastric cancer cell lines SGC7901.The ER-α36 protein expression level and the invasion of constructed cells were detected by Western blot and transwell. The target gene of miR-143 was predicted by bioinformatics tools.Luciferase reporter assay was carried out to confirm the predicted target gene. Results The infection efficiency of the lentivirus titers of LV-miR-143 and LV-anti-miR-143 were over 80% shown by the green fluorescence. The ER-α36 expression level,the cell invasion in LV-miR-21 group were significantly lower than those in LV-anti-miR-21 group(P<0.05). Conclusions miR-143 plays an important role in the negative control of gastric cancer invasion by the regulation of ER-α36.
