Expression of miR-207 is up-regulated in renal and urine of rats with renal fibrosis
- VernacularTitle:miR-207在大鼠纤维化肾组织及尿液中表达上调
- Author:
Bian-Hua SHI
1
;
Jia-Zeng SUN
;
Shang ZHANG
;
Juan SHI
Author Information
1. 中国医学科学院 北京协和医学院 基础医学研究所 医学分子生物学国家重点实验室
- Keywords:
renal fibrosis;
miR-207;
NRK-52E
- From:
Basic & Clinical Medicine
2018;38(3):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression of miR-207 in urine samples and kidney of rat renal fibrosis (renal fibrosis, RF) model and its function was investigated. Methods The rats were divided into two groups,including one undergoing ligation of the right ureter as UUO rat model,the other just under going the same surgery but no ligation for the ureters and as a shamed rat model.Real-time PCR(RT-qPCR) was used to detect the expression of miR-207 in kidney and urine of rat renal fibrosis model. The function of miR-207 was investigated with transfection of miR-207 mimics and inhibitor to the rat renal tubular epithelial NRK-52E cells. Results miR-207 expression in urine and kidney tissues of rat renal fibrosis model was upregulated(P＜0.05). miR-207 in-creased the expression of gene related to fibrosis in rats renal tubular epithelial NRK-52E cells. Conclusions miR-207 may play an important role in renal fibrosis and provide a potential method for the diagnosis of renal fibrosis.
