Overexpression of human telomerase reverse transcriptase inhibits myocardial apoptosis induced by hypoxia/reoxigenation in rats
- VernacularTitle:人端粒酶反转录酶过表达抑制低氧/复氧大鼠心肌细胞的凋亡
- Author:
Ping-Shan WANG
1
;
Yang LIU
Author Information
1. 桂林医学院附属医院 心外科
- Keywords:
TERT;
myocardial cell;
hypoxia/reoxigenation injury
- From:
Basic & Clinical Medicine
2018;38(2):224-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human telomerase reverse transcriptase (TERT) on myocardial apoptosis induced by hypoxia/reoxigenation (Hypo/Ro) in rats. Methods Adenovirus mediated transfection of TERT gene into myocardial cells of neonatal rats, lentivirus-mediated siRNA silencing the TERT. To investigate the protective effects of human telomerase reverse transcriptase on myocardial hypoxia/reoxigenation injury in rats. Results The apoptosis rate of Hypo/Ro group was significantly higher than that of the control group. Compared with the Hypo/Ro group, TERT over-expression group significantly reduced the rate of apoptosis, and over-expression of TERT decreased the expression of CytC gene and increased the expression of P21 gene in cardiomyo-cytes (P＜0.05).Conclusions Over expression of telomerase reverse transcriptase inhibits myocardial apoptosis induced by Hypo/Ro. The mechanism may be explained by the regulation to the expression of P21 and CytC genes.
