Curcumin inhibits apoptosis of HK-2 cell line induced by cisplatin
- VernacularTitle:姜黄素抑制顺铂诱导人肾小管上皮细胞系HK-2凋亡
- Author:
Wen-Xiu LYU
1
;
Wen-Tao WU
;
Shi-Mei ZHENG
;
Lin-Hu XIE
;
Bo-Ping DING
Author Information
1. 皖南医学院 临床医学院 安徽 芜湖 241000
- Keywords:
curcumin;
cisplatin;
HK-2;
cell apoptosis
- From:
Basic & Clinical Medicine
2018;38(2):185-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether curcumin could inhibit cisplatin-induced apoptosis of HK-2 cells. Methods Using MTT methods to observe inhibitory effects of curcumin and cisplatin on the proliferation of HK-2 cell. Detecting caspases expression after curcumin and cisplatin treatment by Western blot. Results Cisplatin induced cell apoptosis, and the effect was dose-dependent(P<0.05).Under the synergistic effect of curcumin and cisplatin on HK-2 cell, the number of apoptosis decreased(P<0.05). In the same time the expression of Bcl-2 protein increased (P < 0.05) and the expression of Bcl-2 protein decreased (P < 0.05). Conclusions Cisplatin enhances the apoptosis of HK-2 cell in a dose-dependent manner, and is inhibited by curcumin. Curcumin can antagonize cisplatin-induced apoptosis in HK-2 by regulating the expressions of Bax and Bcl-2 protein.