Transfection with lentivirus carrying Ifi204 genes up-regulates p204 expression of the aortic vascular fibroblasts in rats
- VernacularTitle:转染携带Ifi204基因的慢病毒上调大鼠主动脉成纤维细胞的p204表达
- Author:
Hui HU
1
;
Qiang WU
;
Mao-Bo TIAN
Author Information
1. 贵州省六盘水市人民医院 老年病科
- Keywords:
interferon-induced protein;
p204;
lentivirus vector;
transfection;
fibroblast;
rats
- From:
Basic & Clinical Medicine
2018;38(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of transfection with lentivirus carrying Ifi204 genes on the expression of p204 in rat vascular adventitial fibroblasts. Methods Culturing the vascular adventitial fibroblasts of rat aortic and then conducting immunocytochemistry to identify the type and purity of these rat fibroblasts. The vascular adventitial fibroblasts of rat aortic cultured were transfected with lentivirus vector system carrying Ifi204 genes (Ifi204 group), the fibroblasts which has been transfected with the empty vector lentivirus particles as the control group (group C), and the untreated fibroblasts as the blank group (group N). The expressions of mRNA and protein of p204 in the fibroblasts were detected by realtime fluorescent quantitative PCR and Western blot test separately. Results There was expression of p204 in group N. Compared with group N and group C, mRNA and protein expression of p204 in the Ifi204 group had been all upregulated (P＜0.01). Conclusions There is structural expression of p204 in vascular adventitial fibroblasts of rat aortic. Transfection with lentivirus carrying Ifi204 genes can upregulate the expression of p204 in vascular adventitial fibroblasts.
