Effect of fluorofenidone on renal interstitial fibrosis in rats with unilateral ureteral obstruction
10.11817/j.issn.1672-7347.2018.05.009
- VernacularTitle:氟非尼酮对单侧输尿管梗阻大鼠肾间质纤维化的疗效观察
- Author:
Wenqing TAN
1
;
Wei WANG
;
Xuan ZHENG
;
Jiying CHEN
;
Xiangning YUAN
;
Fangfang ZHANG
;
Shuting WANG
;
Lijian TAO
Author Information
1. 中南大学湘雅医院肾内科
- Keywords:
renal interstitial fibrosis;
fluorofenidone;
collagen type Ⅰ;
collagen type Ⅲ;
connective tissue growth factor;
platelet-derived growth factor;
α-smooth muscle actin
- From:
Journal of Central South University(Medical Sciences)
2018;43(5):511-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of fluorofenidone on renal interstitial fibrosis in rats with unilateral ureteral obstruction (UUO) and to observe the effect of fluorofenidone on the expressions of collagen type Ⅰ (Col Ⅰ),collagen type Ⅲ (Col Ⅲ),α-smooth muscle actin (α-SMA),connective tissue growth factor (CTGF),platelet derived growth factor (PDGF) in the renal tissues of UUO rats.Methods:Male Sprague-Dawley (SD) rats were randomly divided into a sham-operated group,a UUO group,and a flurofenidone group (n=5).UUO model was induced by ligating the left ureter in rats.The rats were treated with 125 mg/(kg.d) fluorofenidone by gastric gavage in the fluorofenidone group at 24 h before the operation,and the rats were treated with the identical dose of 0.5％ sodium carboxyl methyl cellulose (CMC-Na) in the other 2 groups.The rats were sacrificed at 14 days after UUO.Pathological changes of the renal tissue were observed by HE and Masson staining,the mRNA expressions of Col Ⅰ,Col Ⅲ,α-SMA,PDGF and CTGF were detected by real-time PCR,and the protein expressions of Col Ⅰ,Col Ⅲ,PDGF and CTGF were detected by immunohistochemical staining.Results:The renal interstitial damage index,relative collagen area and mRNA and protein expressions of Col Ⅰ and Col Ⅲ in the renal tissues of the rats in the UUO group significantly increased (P＜0.05),and fluorofenidone could reduce these indexes (P＜0.05).Compared with the sham-operated group,the protein expressions ofα-SMA,PDGF,CTGF and the mRNA expressions of PDGF and CTGF in the renal tissues of the rats in the UUO group were increased,but fluorofenidone could decrease the protein expressions of α-SMA,PDGF,CTGF and the mRNA expressions of PDGF and CTGF (P＜0.05).Conclusion:Fluorofenidone (125 mg/kg·d) could attenuate renal interstitial fibrosis through inhibition offibroblast proliferation,myofibroblastic activation,PDGF and CTGF expression.
