Mechanism for synergistic effect of IRF4 and MITF on tyrosinase promoter
10.11817/j.issn.1672-7347.2018.05.001
- VernacularTitle:IRF4与MITF协同作用于络氨酸酶启动子的相关机制
- Author:
Jian SONG
1
;
Xueming LIU
;
Jiada LI
;
Huadie LIU
;
Zhen PENG
;
Hongsheng CHEN
;
Lingyun MEI
;
Chufeng HE
;
Yong FENG
Author Information
1. 中南大学湘雅医院耳鼻咽喉科
- Keywords:
interferon regulatory factor 4;
microphthalmia-associated transcription factor;
tyrosinase;
transcription activation;
synergic effect
- From:
Journal of Central South University(Medical Sciences)
2018;43(5):461-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism for the synergistic effect of interferon regulatory factor 4 (IRF4) and microphthalmia-associated transcription factor (MITF) on tyrosinase (TYR)promoter.Methods:The synergistic transcriptional effect,subcellular localization,and protein-protein interaction for IRF4 and MITF were observed by luciferase assay,immunofluorescence,GST-pull down,and co-immunoprecipitation,respectively.Results:IRF4 and MITF proteins were co-expressed in the cell nucleus.IRF4 augmented the transcriptional function of MITF (but not the mutant MITF) to activate the expression of the TYR promoter,but with no effect on other MITF-specific target promoters.IRF4 alone did not affect TYR promoter significantly.No direct interaction between the two proteins was noted.Conclusion:IRF4 and MITF exert a specifically synergistic effect on activation of TYR promoter through IRF4-mediated upregulation of transcriptional function of MITF.This synergistic effect is mainly regulated by MITF;DNA might be involved in the interaction between the two proteins.
