Properties of decellularized rabbit carotid artery with two different cross-linked technologies: A comparative study
10.11817/j.issn.1672-7347.2018.04.017
- VernacularTitle:两种交联技术处理的兔去细胞颈动脉性能比较
- Author:
Lei HUANG
1
;
Feng KUANG
;
Demiao KONG
;
Xinmin ZHOU
Author Information
1. 中南大学湘雅二医院心血管外科
- Keywords:
decellularization;
photo-oxidation;
procyanidin;
anti-calcification;
cross-link
- From:
Journal of Central South University(Medical Sciences)
2018;43(4):441-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the properties between decellularized rabbit carotid artery with different cross-linked technologies.Methods:The decellularized rabbit carotid arteries were randomly divided into a photo-oxidation group and a procyanidins group.One group was cross-linked with photo-oxidation and the other group was cross-linked with procyanidins.The in vitro or in vivo properties of the two groups were evaluated by testing heat-shrinking temperature,max tensile strength and the max elongation or by testing tissue structure,inflammatory reaction and calcification degree.Results:The heat-shrinking temperature,max tensile strength and the max elongation were similar in the two groups (P＞0.05).The tissue structure and inflammatory reaction were also similar in the two groups.Mthough the result of Von-Kossa calcium salt stain was slightly different,the calcium content was lower in the procyanidins group than that in the photo-oxidation group (P＜0.05).Conclusion:The grafts by two cross-linked technologies show excellent mechanical capability,lower immunogenicity,good biological stability and anti-calcification ability.The procyanidins group shows a better anti-calcification property than the photo-oxidation group.
