Synthetic and Adenovirus Delivered Small Interference RNA Pools Targeting Conserved Regions of Foot-and-Mouth Disease Virus.
10.4167/jbv.2010.40.4.199
- Author:
Su Mi KIM
1
;
Kwang Nyeong LEE
;
Young Joon KO
;
Yi Seok JOO
;
Hyun Soo KIM
;
Jong Hyeon PARK
Author Information
1. Foreign Animal Disease Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Korea. parkjhvet@korea.kr
- Publication Type:Original Article
- Keywords:
siRNA;
Pool;
Adenovirus;
Antiviral effects
- MeSH:
Adenoviridae;
Animals;
Asia;
Conserved Sequence;
Foot-and-Mouth Disease;
Foot-and-Mouth Disease Virus;
RNA;
RNA, Small Interfering;
Vaccination;
Virus Replication
- From:Journal of Bacteriology and Virology
2010;40(4):199-206
- CountryRepublic of Korea
- Language:English
-
Abstract:
Foot-and-mouth disease (FMD) is an economically significant animal disease because of the speed of its transmission. Routine vaccination may not be effective for early protection in an outbreak situation. Small interfering RNA (siRNA) can be used as a rapid, effective, and an alternative antiviral approach. In this study, we screened 15 synthetic siRNAs to inhibit FMD virus replication in IBRS-2 cells and selected 10 siRNA sequences. Furthermore, we produced 7 adenoviruses expressing shRNA targeting conserved regions of FMDV, such as a leader sequence and nonstructural protein regions, and showed their antiviral effects. We compared the antiviral effects among them and compared between synthetic siRNAs and adenovirus-delivered siRNAs. In particular, the most efficient siRNA, 3C2, was the conserved sequence in the O, A, Asia 1, and C serotypes of FMDV and was located in the predicted loop structure. The pool of sequences including 3C2 and recombinant adenoviruses could be applied for multiple siRNAs and protection in a broad range of cells and animals.
