Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis.
10.3347/kjp.2004.42.3.145
- Author:
Soo Ung LEE
;
Sun HUH
- Publication Type:Brief Communication ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Clonorchis sinensis;
ribosomal DNA;
mitochondrial DNA;
China;
Korea
- MeSH:
Animals;
Base Sequence;
China;
Clonorchis sinensis/enzymology/*genetics;
Comparative Study;
DNA, Helminth/chemistry/*genetics;
DNA, Mitochondrial/chemistry/*genetics;
DNA, Ribosomal/chemistry/genetics;
DNA, Ribosomal Spacer/chemistry;
Electron Transport Complex IV/genetics;
Genetic Markers;
Korea;
Molecular Sequence Data;
RNA, Ribosomal, 18S/genetics;
Research Support, Non-U.S. Gov't;
Sequence Alignment;
Species Specificity;
*Variation (Genetics)
- From:The Korean Journal of Parasitology
2004;42(3):145-148
- CountryRepublic of Korea
- Language:English
-
Abstract:
We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.
