Angiogenic ability of 3 different tissues-derived mesenchymal stem cells on endothelial progenitor cells
10.11817/j.issn.1672-7347.2018.02.014
- VernacularTitle:3种不同组织来源的间充质干细胞促内皮祖细胞血管形成作用的比较
- Author:
Siqi WANG
1
;
Haiyuan LU
;
Lamei CHENG
Author Information
1. 中南大学基础医学院生殖与干细胞工程研究所
- Keywords:
human bone marrow derived mesenchymal stem cells;
human adipose derived mesenchymal stem cells;
human umbilical cord derived mesenchymal stem cells;
endothelial progenitor cells;
angiogenesis;
vascular stability
- From:
Journal of Central South University(Medical Sciences)
2018;43(2):184-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the ability between bone marrow-derived mesenchymal stem cells (MSCs) (BM-MSCs) and adipose-derived MSCs (AD-MSCs) or umbilical cord-derived MSCs (UC-MSCs) on promotion of vessels formation and vessds stabilization relevant to the functions of EPCs.Methods:In vitro,co-culture blood vessel test was performed to compare the angiogenic ability between BM-MSCs,AD-MSCs or UC-MSCs.In vivo,angiogenic assay dependent on basement membrane matrix Matrigel and immunohistochemistry were performed to compare the ability of vessels formation functions between BM-MSCs and AD-MSCs or UC-MSCs.Results:The lengths and dots of vascular structures formed by EPCs on AD-MSCs layer are greater than those by EPCs on BM-MSCs layer and UC-MSCs layer in angiogenic assay in vitro.The stability of the capillary-like structures formed by EPCs with AD-MSCs on Matrigel was more stable than that by the BM-MSCs,UC-MSCs or EPCs.AD-MSCs and EPCs could form abundant functional vessels with blood perfusion in Matrigelin vivo;UC-MSCs and EPCs could form a few functional vessels with blood perfusion in Matrigelin vivo;BM-MSCs and EPCs could form broken vessels with hemocytes leakage in Matrigel in vivo.Conclusion:AD-MSCs have the stronger ability to promote the angiogenesis and stabilize the vessels compared with BM-MSCs or UC-MSCs ex vivo and in vivo.
