Expression of H19 long non-coding RNA and ZEB1 in the trophoblast of women with spontaneous abortion
10.11817/j.issn.1672-7347.2018.02.013
- VernacularTitle:自然流产绒毛组织中长链非编码RNA H19及ZEB1的表达
- Author:
Lian HU
1
;
Hong ZENG
;
Nenghui LIU
Author Information
1. 中南大学湘雅医院生殖医学中心
- Keywords:
spontaneous abortion;
H19;
zinc finger E-box-binding protein 1
- From:
Journal of Central South University(Medical Sciences)
2018;43(2):179-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of H19 long non-coding RNA (IncRNA) and zinc finger E-box-binding protein i (ZEB1) in the trophoblast of women with spontaneous abortion.Methods:A total of 20 women underwent miscarriage were enrolled as a miscarriage group,while 20 women underwent artificial abortion were enrolled as a control group.Reverse transcriptional fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of iH19 lncRNA and miR-200,and Western blot was used to detect the expression of ZEB1.Results:Compared to the control group,a tendency of decreased H19 was detected in the miscarriage group,with no significant difference (1.28±0.08 vs 1.66±0.21,P=0.091).There was no significant difference in miR-200 between the 2 groups (0.75±0.35 vs 0.58±0.33,P=0.0.533).The expression of ZEB 1 was significantly decreased in the miscarriage group compared to that in the control group (0.22±0.03 vs 0.41±0.04,P=0.0003).The expression of i19 lncRNA and ZEB1 was positively correlated (r=0.529,P=0.0005),and the correlation between H19 lncRNA and miR-200 had no statistical significance (r=0.0293,P=0.858).The correlation between miR-200 and ZEB1 also had no statistical significance (r=-0.132,P=0.416).Conclusion:Down-regulation of ZEB1 in the trophoblast might be related to miscarriage.H19 lncRNA may regulate the expression of ZEB1.
