Bactericidal effect of ozonated camellia oil on Staphylococcus aureus in vitro
10.11817/j.issn.1672-7347.2018.02.005
- VernacularTitle:臭氧化山茶油对金黄色葡萄球菌的体外杀伤效果
- Author:
Yaping XIANG
1
;
Jianyun LU
;
Feifeng LI
;
Jian HUANG
;
Caifeng YANG
;
Zhibing FU
;
Lihua GAO
Author Information
1. 中南大学湘雅三医院皮肤科
- Keywords:
ozonated camellia oil;
Staphylococcus aureus;
in vitro;
plate count method;
turbidimetry
- From:
Journal of Central South University(Medical Sciences)
2018;43(2):139-142
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore a new method for detecting the bactericidal effect of oiling agent in vitro,and to determine the disinfectant effecacy ofozonated camellia oil on Staphylococcus aureus.Methods:Suspension of Staphylococcus aureus was prepared and innoculated on the LB plate by plate scribing method.After culture overnight,21 bacterial monoclones with the same diameter were selected and divided into 3 groups:A negative control group,a baseoil (camellia oil) group and an ozonated camellia oil group.We used a ring to isolate the single clone and added oil inside the ring,cultured the whole plate over night,picked out each single clone (with gel) to 5 mL LB medium and cultured it for 12 h.The final concentration of the LB medium was detected by plate count method and turbidimetry.Results:According to the plate count method and turbidimetry,the bacterial concentration in the ozonated camellia oil group was lower than that in the negative control group and base oil group Conclusion:Bacterial monoclone culture method shows that ozonated camellia oil can significantly kill Staphylococcus aureus,and this method is an effective method for evaluating the bactericidal function of the oiling agent in vitro.
