Effects of concentrated growth factors on proliferation and osteogenic differentiation in Beagle adipose-derived stem cells
10.11817/j.issn.1672-7347.2018.01.001
- VernacularTitle:自体浓缩生长因子对比格犬脂肪干细胞体外增殖及成骨向分化的影响
- Author:
Xiangyu MA
1
;
Lidan DING
;
Shijun TANG
;
Ting LI
;
Jinlong PEI
;
Yong LI
Author Information
1. 重庆医科大学附属口腔医院
- Keywords:
concentrated growth factor;
adipose-derived stem cells;
proliferation;
osteogenic differentiation
- From:
Journal of Central South University(Medical Sciences)
2018;43(1):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of concentrated growth factor (CGF) on proliferation and differentiation in Beagle adipose-derived stem cells (ADSCs).Methods:ADSCs were isolated from adipose tissue of healthy Beagles and cultured.The multidirectional differentiation potential of ADSCs was identified.The ADSCs were assigned to a CGF group and a control group.The rate of proliferation was analyzed by CCK-8 assay.The osteogenic differentiation capability was detected by ALP staining after the osteoinduction.Bone formationrelated gene expression was detected by RT-PCR.Results:CGF promoted the proliferation of ADSCs in vitro.ADSCs in the CGF group showed higher level of ALP activity than that in the control group (P＜0.05).CGF stimulated the expression of the genes associated with osteogenesis,such as Col-I and Runx2.Conclusion:CGF can promote the proliferation and osteogenic differentiation in ADSCs in vitro.
