Role of high mobility group protein BI in IL-1α-induced endothelial cell senescence
10.11817/j.issn.1672-7347.2017.12.002
- VernacularTitle:高迁移率族蛋白BI在IL-1α诱导的内皮细胞衰老过程中的作用
- Author:
Ting FANG
1
;
Yapei LI
;
Zhouyangfan PENG
;
Zhen ZHANG
;
Fengyuan CHEN
Author Information
1. 中南大学湘雅三医院心内科
- Keywords:
vascular endothelial cell;
senescence;
inflammation;
IL-1α;
high mobility group protein 1
- From:
Journal of Central South University(Medical Sciences)
2017;42(12):1361-1366
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of interleukin-1α (IL-1 α) on the senescence of human umbilical vein endothelial cells (HUVECs) and the function of high mobility group protein 1 (HMGB 1).Methods:HUVECs were randomly divided into a control group,a IL-1α group (10 ng/mL IL-1α),a HMGB 1 group (100 ng/mL HMGB 1),and a HMGB 1 +IL-1α group (100 ng/mL of HMGB 1 plus 10 ng/mL of IL-lα).Senescence associated β-galactosidase (SA β-gal) staining was used to assess the number of senescent cells,Western blot were performed to detect the protein levels of silent information regulator 1(SIRT1),and quantitative real-time PCR (qRT-PCR) was used to detect the mRNA levels of p53,p21 and p 16.Restlts:Compared with the control group,the number of SA β-gal positive cells were significantly increased in the IL-1α group (P＜0.05),while the expression of SIRT1 protein significantly decreased (P＜0.01).Compared with the IL-1 α group,the expression of SA β-gal positive cells in the HMGB 1+IL-1α group was decreased and the mRNA levels of p21 and p53 were down-regulated (all P＜0.05),however,there was no statistical significance in the mRNA expression ofp16 (P＞0.05).Conclusion:IL-1α can induce the senescence of HUVECs,and HMGB1 may inhibit IL-1α-induced endothelial cell senescence via p53-p21 pathway.
