Study on Stability of Mixture Reference Solution of Strychni Alkaloids
10.13359/j.cnki.gzxbtcm.2018.02.025
- VernacularTitle:马钱子生物碱混合对照溶液稳定性研究
- Author:
Fei-Fei WANG
1
;
Shou-Hai WU
;
Ming-Juan WANG
;
Yu-Mei ZHANG
;
Shuang-Cheng MA
Author Information
1. 中国食品药品检定研究院
- Keywords:
Semen Strychni;
brucine;
strychnine;
quality control
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2018;35(2):328-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective Brucine and strychnine monomer reference substance as extremely toxic substance had potential threat during transportation and utilization. In this study we investigated the homogeneity, stability and assignment accuracy of the mixture reference solution of strychnine and brucine, so as to provide reference for the quality control of extremely toxic chemical reference substances for Chinese medicine. Methods Following the assay in Chinese Pharmacopoeia volume I (2015), we prepared the mixture reference solution of brucine and strychnine, and investigated the solvents and the concentration of mixutre reference solution. The stability test lasted for 12 months. F-test was used for heterogeneity assay. Three researchers were involved for collaboration. Results Methanol and chloroform solution were selected as the solvents for the stability test. Results showed the difference was not statistically significant among various mixture solutions. The results of value assignment were 0.14 mg/mL for strychnine (sR = 0.5％)and 0.10 mg/mL for brucine (sR = 1.0％). The stability of mixture solution were better under the conditions of methanol solution at 4 ℃ or -20 ℃. Conclusion The results provide a possible way to develop the mixture solution in place of the monomer reference, and the mixture reference solution is expected for the quality control in the slices of Semen Strychni and its compound preparations.
