Study on the simultaneous measurement of residual level of three pesticides in nelumbinis semen using GC-MS/MS
10.3760/cma.j.issn.1673-4246.2018.10.015
- VernacularTitle:气相色谱-质谱法测定莲子中农药残留的一测多评研究
- Author:
Zhikang TANG
1
;
Minling YU
;
Minfeng ZHU
;
Fudong ZHANG
Author Information
1. 西南科技大学生命科学与工程学院
- Keywords:
Nelumbinis semen;
GC-MS/MS;
Bifenthrin;
Fenpropathrin;
Deltamethrin;
Pesticide residues;
Quantitative analysis of multi-components with a single-marker
- From:
International Journal of Traditional Chinese Medicine
2018;40(10):959-964
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quantitative method for the simultaneous measurement of the residual level of three pesticides in Nelumbinis semen by GC-MS/MS. Methods The samples were extracted by acetonitrile and purify by Cleanert TPH column. The samples were then tested by GC-MS/MS. Information on relative retention time and mass charge ratio was used for qualitative analysis. The peak area obtained by secondary ion MS of bifenthrin (181.1/166.1)was used as the reference peak to calculate the relative correction factor for the peak area of fenpropathrin (265.1/210.1) and deltamethrin (252.9/93.0), to establish a method using bifenthrin as the reference substance to determinate there sidual quantity of three pesticides in Nelumbinis semen by GC-MS/MS. Results When the injection quantity of the sample containing bifenthrin,fenpropathrin and deltamethrin in the range of 0.01-0.1 ng , there was a good linear relationship between the injection quantity and peak are a Limitation of quantification (LOQ) of bifenthrin , fenpropathrin and deltamethrin were 4.321×10-4 ng, 3.435×10-4 ng, 8.913×10-3 ng, respectively. The average recovery rates of bifenthrin, fenpropathrin and deltamethrin were 93.5％, 93.5％ and 93.8％, respectively. Conclusions The method of quantitative analysis of multi-components with a single-marker is simple, quick and accurate. It suitable for the detection of residual quantity of bifenthrin, fenpropathrin and deltamethrin in Nelumbinis semen.
