Study on Luoshi-Neiyi prescription in the expression of E-cadherin in endometriosis model rats
10.3760/cma.j.issn.1673-4246.2018.10.014
- VernacularTitle:罗氏内异方对子宫内膜异位症模型大鼠E钙黏蛋白表达的影响
- Author:
Biyun LUO
1
;
Li LI
Author Information
1. 510405,广州中医药大学
- Keywords:
Endometriosis;
E-cadherin;
Luoshi-Neiyi prescription;
Rats
- From:
International Journal of Traditional Chinese Medicine
2018;40(10):955-958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different doses Luoshi-Neiyi prescription in expression of E-cadherin of endometriosis model SD rats. Methods The female rats with SPF class SD were randomly divided into six groups: high-dose group, medium-dose group, low-dose group, model group, sham operation group and blank group, 15 of each group. Apart from the sham operation group and the blank group, the rats in the remaining group used abdominal cavity injection to establish the model of the endoheterosis rat model. After the successful establishment of the model, the mice were given high, medium,low dose and the sterilization water for 8 weeks. After stopping the drug, the left uterus of the rats was taken from the left uterus of each group in the fourth day of pregnancy to detect the E-cadherin by Western Blotting. Results Compared with the model group, the expression of E-cadherin (0.76 ± 0.26, 0.96 ± 0.33 vs. 0.24 ± 0.05) in the medium-dose and high dose groups significantly incresed (P<0.05). There was no differentence in the mediumdose and highdose groups (P<0.05). Conclusions The medium dose and high dose of Luoshi-Neiyi prescription can up-regulate the expression of E-cadherin in Endometriosis model rats.
