The effects of Xuling-Jiangu formula on the mRNA expression of osteoprotegerin and receptor activator of nuclear factor κB ligand in osteoporosis model rats
10.3760/cma.j.issn.1673-4246.2018.09.013
- VernacularTitle:续苓健骨方对骨质疏松症模型大鼠细胞核因子κB受体活化因子配体及骨保护素基因表达的影响
- Author:
Shengqiang LI
1
;
Sainan CHEN
;
Lihua XIE
;
Bingying XIE
;
Jirong GE
Author Information
1. 350003福州,福建省中医药研究院基础医学研究所,骨质疏松证候基因组学重点研究室
- Keywords:
Osteoporosis;
Bone density;
Osteoprotegerin;
Receptor activator of nuclear factor κB ligand;
Xuling-Jiangu formula;
Rats
- From:
International Journal of Traditional Chinese Medicine
2018;40(9):840-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects and mechanism of Xuling-Jiangu formula on bone mineral density in the osteoporosis model rats.Methods According to the random number table method,50 SD female rats were randomly divided into the sham group,the model group,the low dose group of Xuling-Jiangu formula group,the medium dose group and the high dose of group.In addition to the sham group,the other groups were osteoporosis model.After 30 days,low dose group received intragastric Xuling-Jiangu formula solution 7.5 g/kg;medium dose group and high dose group received 15 and 30 g/kg,respectively.And the sham group and the model group received normal saline 10 ml/kg.After 12 weeks treatment,the bone mineral density of the left tibia was measured by double energy X ray.Estrone,osteocalcin in serum had been detected by Enzyme linked immunosorbent assay (ELISA).The mRNA expression of osteoprotegerin (OPG),receptor activator of nuclear factor κB ligand (RANKL) in lumbar were measured by quantitative real-time PCR.Results The bone mineral density (0.215 ± 0.010 g/cm2,0.222 ± 0.013 g/cm2 vs.0.196 ± 0.016 g/cm2) of the Xuling-Jiangu formula group were significantly higher than that of the model group (P＜0.01 or P＜0.05).The estrone in low,middle and high dose groups showed an upward trend,but there was no significant difference compared with the model group.The OC (87.0 ± 8.9 ng/L vs.100.5 ± 16.8 ng/L) of high dose group was significantly lower than that of the model group (P＜0.05).Compare with the model group,OPG mRNA level (0.97 ± 0.23 vs.0.78 ± 0.17) in high dose group increased (P＜0.05),the RANKL mRNA level decreased significantly (1.12 ± 0.17,0.97 ± 0.38,1.04 ± 0.29 vs.1.31 ± 0.18) in three Xuling-Jiangu formula groups (P＜0.05).Conclusions The bone mineral density of rats with osteoporosis can be improved by treating Xuling-Jiangu formula.It may be related to the increase of mRNA expression of OPG,and the reduction of RANKL.
