The application of HPLC-MS/MS for comprehensively monitor the feeding analysis of Yuanhu analgesic capsules
10.3760/cma.j.issn.1673-4246.2018.07.014
- VernacularTitle:液质联用法监测元胡止痛胶囊原料药的投料分析研究
- Author:
Xiaolin WANG
1
;
Minfeng ZHU
;
Chao LIU
;
Hualing LUO
;
Maohua YUAN
;
Fudong ZHANG
Author Information
1. 614000,四川省乐山市食品药品检验检测中心
- Keywords:
Tetrahydropalmatine;
Imperatorin;
Mass spectrometry;
Yuanhu analgesic capsules;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2018;40(7):639-644
- CountryChina
- Language:Chinese
-
Abstract:
Objective The HPLC-MS/MS is used to comprehensively monitor the feeding conditions of raw materials in Yuanhu analgesic capsules, and the content of the index components can be detected at the same time. Methods The Inertsil ODS-3 (4.6 mm×250 mm, 5 μm) was used with the column temperature 40 ℃, Flow phase: 0.1% formic acid-acetonitrile; the gradient elution program, active ingredients were separated by HPLC, and the Electrospray Ionization Mass (ESI) source was applied and operated in the negative ion mode, and reactions ion monitoring mode (MRM) for quantitative analysis were selected. Results Through analysis and contrast of the medicinal materials, reference substance of primary mass spectrogram showed the same characteristic peak, and the proprietary Chinese medicine can be judged by prescription feeding process. The tetrahydroxene and imperatorin had a good linear relationship in 5.08×10-5-30.45×10-5μg (r=0.999 4), 5.02×10-5-30.09×10-5μg (r=0.999 2). Precision test were 0.99% and 1.14%, the recovery rate were 97.02%-99.66%, 97.62%-99.94%. Conclusions The method is simple, accurate and reliable, high sensitive and fast. It is suitable to monitor the feeding condition and quality of yuanhu analgesic capsules.
