Study on the alkaloids in the different parts of Aconitum paniculigerum Nakai var.wulingense (Nakai) by HPLC-MS

VernacularTitle:液质联用法分析雾灵乌头不同部位生物碱成分研究
Author: Xiaoyu GUO ¹ ; Jiamei ZHANG ; Qinghua QUAN ; Ruifang JI ; Peng TAN ; Yonggang LIU
Author Information

1. 102488,北京中医药大学中药学院
Keywords: Aconitum; Alkaloids; Mass spectrometry
From: International Journal of Traditional Chinese Medicine 2018;40(7):633-638
CountryChina
Language:Chinese
Abstract: Objective To analyze the alkaloids in the different parts of Aconitum wulingense by HPLC-ESI-Trap-MS. Methods The Agilent XDB-C18(250 mm×4.6 mm, 5 μm) column with gradient elution of 0.1% solvent (A)-acetonitrile(B), at a flow rate of 1 ml/min was used. The column temperature was set at 30 ℃. The MS analysis was based on positive ions mode. Results In the roots, a total of 61 diterpenoid alkaloids were discovered, among which 46 were identified. In the stems, 38 alkaloids have been found, among which 33 alkaloids were identified and 27 were the same with the roots. In the leaves, 18 alkaloids have been detected and 8 were the same with the roots. Conclusions The method is accurate, reliable and efficient, and is suitable for rapid identification of ingredients in Aconitum wulingense, which provides a reference for the development and utilization of Aconitum wulingense and clarify its efficacy and material basis.