Study on quality standard of Bushen-Tongluo granules
10.3760/cma.j.issn.1673-4246.2018.04.013
- VernacularTitle:补肾通络颗粒质量标准研究
- Author:
Haiying ZHANG
1
;
Meiling WANG
;
Zixuan DU
;
Xingbin YIN
;
Jian NI
Author Information
1. 100029,北京中医药大学中药学院
- Keywords:
Chromatography;
thin layer;
Chromatography;
high pressure liquid;
Quality control;
Bushen-Tongluo granules;
Naringin;
Paeoniflorin
- From:
International Journal of Traditional Chinese Medicine
2018;40(4):345-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard for Bushen-Tongluo granules.Methods Drynariae rhizoma, Paeoniae radix alba, Cyathulae radix, Chuanxiongin rhizome were identified by thin layer chromatography (TLC), and the content of naringin and paeoniflorin was determined by high performance liquid chromatography(HPLC) with chromatographic column Agilent C18 (4.6 mm×250 mm, 5 μm), as the mobile phase was acetonitrile-0.1% phosphoric acid and the scan wave length were 230 and 283 nm. The column temperature was 30 . The flow rate ℃ was 1.0 ml/min. Results TLC could identify Drynariae rhizoma , Paeoniae radix alba, Cyathulae radix, Chuanxiongin rhizome effectively. Under the condition, there was a good linear relationship when the content of naringin was in 6.337 0-50.695 7 μg. There is also a good linear relationship when the content of paeoniflorin was in 26.065 8-130.328 8 μg. The average recovery rate of naringin was 97.13% and RSD was 1.19%.The average recovery rate of paeoniflorin was 96.61% and RSD was 1.51%. Conclusions The established methods are simple, specific, reproducible, and sensitive, and they can be used for the quality control of Bushen-Tongluo granules.
