Syphacia obvelata: A New Hope to Induction of Intestinal Immunological Tolerance in C57BL/6 Mice.
10.3347/kjp.2017.55.4.439
- Author:
Niloofar TAGHIPOUR
1
;
Nariman MOSAFFA
;
Mohammad ROSTAMI-NEJAD
;
Mohamad Mohsen HOMAYONI
;
Esmaeil MORTAZ
;
Hamid Asadzadeh AGHDAEI
;
Mohammad Reza ZALI
Author Information
1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
- Publication Type:Brief Communication
- Keywords:
Syphacia obvelata;
T regulatory cell;
Foxp3;
C57BL/6;
inflammatory disorder;
intestinal tolerance
- MeSH:
Animals;
Flow Cytometry;
Helminths;
Hope*;
Immune System;
Interleukin-10;
Interleukin-17;
Lymph Nodes;
Mice*;
Oxyuroidea*
- From:The Korean Journal of Parasitology
2017;55(4):439-444
- CountryRepublic of Korea
- Language:English
-
Abstract:
The ability of nematodes to manipulate the immune system of their host towards a Th2 and T regulatory responses has been proposed to suppress the inflammatory response. Clinical trials have proposed a useful effect of helminth infections on improvement of inflammatory disorders. In this study, we investigated the immunomodulatory effect of Syphacia obvelata infection to induce intestinal tolerance in C57BL/6 mice. Mice were infected through the cagemates with self-infected BALB/c mice. Four weeks post-infection, expression levels of IFN-γ, TNF-α, IL-17, and IL-10 were assessed in the supernatant of mesenteric lymph node (MLN) culture. Foxp3⁺Treg were measured in MLN cells by flow cytometry. In the S. obvelata-infected group, the percentage of Tregs (5.2±0.4) was significantly higher than the control (3.6±0.5) (P<0.05). The levels of IL-10 (55.3±2.2 vs 35.2±3.2), IL-17 (52.9±3.8 vs 41±1.8), IFN-γ (44.8±4.8 vs 22.3±2.3) and TNF-α (71.1±5.8 vs 60.1±3.3) were significantly increased in infected mice compared to the control group (P<0.05). The above results showed the potential effects of S. obvelata to induce intestinal tolerance. Therefore, it seems that S. obvelata may increase the immunological suppressive function in the intestinal tract.
