Simultaneous determination of isoquercetin and astragalin in Moringa oleifera leaves prescription-a prescription for treatment of constipation by HPLC
10.3760/cma.j.issn.1673-4246.2018.02.014
- VernacularTitle:HPLC法测定辣木叶通便复方中异槲皮苷、紫云英苷含量
- Author:
Aoxue LIU
1
;
Wenhong GU
;
Mengwei LI
;
Xinjing CHEN
;
Jun HE
;
Xingfen LONG
;
Yang LIU
Author Information
1. 102488,北京中医药大学中药学院
- Keywords:
Chromatography;
high pressure liquid;
Chemical quantitative analysis;
Moringa oleifera;
Relaxing bowels compounds
- From:
International Journal of Traditional Chinese Medicine
2018;40(2):153-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for simultaneous determination of Isoquercitrin and astragalin in Moringa oleifera leaves prescription. Methods By using HPLC method with Cosmosil-C18 column (4.6 mm × 250 mm, 5 μm) and the column temperature was 40 degrees Celsius, the mobile phase contains a 0.1％phosphoric acid in water(A) and acetonitrile(B), the detection wavelength was set at 350nm by UV detector and the flow rate was 1.3 ml/min. Results The Isoquercitrin and astragalin was better separated in 30 minutes, and Isoquercitrin had a good linear correlation at the range of 0.24-4.73 μg, while astragalin was 0.11-2.19 μg, the average spiked recoveries of isoquercetin and astragalin were 99.09％ (RSD=0.60％) and 99.08％ (RSD=1.37％), respectively. Conclusions The proposed method is simple,accurate,and repeatable and can be used for quality control as well as a powerful tool to evaluate the processing of Moringa oleifera leaves. It can provide the reference basis and data support for the determination of content of Moringa oleifera health products.
