Effects of psoralen isoflavones on the treatment of PC12 cells injured by Aβ
10.3760/cma.j.issn.1673-4246.2018.02.013
- VernacularTitle:补骨脂异黄酮对β-淀粉样蛋白损伤PC12细胞的影响
- Author:
Juncen LIU
1
;
Zhibo ZHANG
;
Haiyang LIU
;
Guoliang LIU
;
Jiazhi WANG
;
Fang GENG
;
Ning ZHANG
Author Information
1. 黑龙江中医药大学药学院
- Keywords:
PC12 cells;
Alzheimer disease;
Amyloid;
Psoralen isoflavones
- From:
International Journal of Traditional Chinese Medicine
2018;40(2):148-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of psoralen isoflavone on the treatment of PC12 cells injured by Aβ and the mechanism on the effect of the psoralen isoflavones on the expression of related proteins. Methods The PC12 cells were divided into blank group, model group, E2 group, and psoralen isoflavones group by random number table method, In addition to the blank group the rest of each group culture medium were added 20μmol/L of Aβ25-35 modeling, The E2 group was added to the 10-3μmol/L oestrogen and psoralen isoflavones group for the intervention of 102-10-6 μmol/L.The proliferation rate of PC12 cells was detected by MTT assay, and the expression of APP, BACE1, ERβ, p-ERK and Aβ protein was detected by Western Blot. Results Compared with the model group, the proliferation of PC12 cells induced by 10-1μmol/L of psoralen isoflavone increased (101％ vs. 52％, P<0.01); The expression of p-ERK (0.751± 0.066 vs. 0.364 ± 0.015), ERβ(0.756 ± 0.105 vs. 0.337 ± 0.045) increased significantly (P<0.01); APP (0.382 ± 0.039 vs. 0.479 ± 0.015), BACE1 (0.517 ± 0.024 vs. 0.622 ± 0.029), Aβ (0.430 ± 0.032 vs. 0.581 ± 0.030) expression amount were significantly lower (P<0.05). Conclusions Psoralen isoflavones have a certain therapeutic effect on PC12 cells injured by Aβ.
