A potential role of macrophage phenotypes in the early evaluation of the effects of combined Yersinia Pestis and Brucella bovis vaccine immunization
10.13220/j.cnki.jipr.2018.01.007
- VernacularTitle:巨噬细胞表型在鼠疫杆菌和牛布鲁氏杆菌联合免疫效果早期评价中的潜在作用
- Author:
Shuai-Jie DOU
1
,
2
;
Ge LI
;
Jia-Cheng ZHANG
;
Yi-Qiong LIU
;
Yan-Ling ZHANG
;
Chun-Mei HOU
;
He XIAO
;
Gen-Cheng HAN
Author Information
1. 230032合肥,安徽医科大学研究生学院
2. 100850北京,军事科学院军事医学研究院军事认知与脑科学研究所天然免疫研究室
- Keywords:
combined immunization;
Yersinia pestis vaccine;
Brucella bovis vaccine;
auti-infectious agents;
macrophage phenotype;
CD8+T cells
- From:
Journal of International Pharmaceutical Research
2018;45(1):37-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective In emergency situations where simultaneous immunization by multiple vaccines are required,how to rapidly evaluate the effect of combined immunization is an urgent issue that needs to be solved.This study aimed to investigate the po-tential role and application value of the phenotypic changes of macrophages in rapid evaluation of the effect of combined Yersinia pestis and Brucella bovis vaccine immunization at early stage.Methods Y.pestis and B.bovis vaccines were injected into mice alone or in combination to establish animal models.The changes of the macrophage phenotypes(M1 or M2 polarization)and the CD8+T cell pheno-types and functions were detected in the early(4 d)and the late(14 d)stage of the immunization,respectively.The effect of the immuno-phenotype of macrophages at early stage on the function of CD8+T cells at late stage was analyzed.Results The co-immunization by Y.pestis and B.bovis vaccines led to the attenuation of the M1-polarization of macrophages at early stage,which were marked by de-creased expression of CD16/32 and increased expression of Detectin-1 on cell surface as well as decreased expression of IL-12 and in-creased expression of IL-4 inside the macrophage,in comparison with single vaccine groups,suggesting an interference between the two vaccines.Meanwhile,the activity of CD8+T cells(including the ratio of CD8+CD69+T,CD8+IFN-γ+T and CD8+GranzymeB+T cells) in combined immunization group showed similar tendency to the attenuated phenotypic M1-polarization of macrophages. Conclusion The phenotype of macrophages at the early stage of the co-immunization by Y.pestis and B.bovis vaccines showed consistency with the phenotype and function of CD8+T cells at late stage.It might give us some hint about the possibility of utilizing the phenotypic changes of macrophages to rapidly evaluate the effect of the co-immunization at early stage.
