Process optimization and component analysis of extracting seed oil from Schisandra sphenanthera by supercritical CO2
10.13220/j.cnki.jipr.2017.10.016
- VernacularTitle:超临界CO2萃取南五味子种子油的工艺优化及其成分分析
- Author:
Min CHENG
1
;
Shuai WEN
;
Xu-Hua LIANG
;
Yi-Lin ZHANG
;
Ting-Ting PAN
;
Xiu-Ning WU
Author Information
1. 商洛学院生物医药与食品工程学院
- Keywords:
response surface methodology;
supercritical CO2extraction;
Schisandra sphenanthera seed oil;
GC-MS
- From:
Journal of International Pharmaceutical Research
2017;44(10):988-994
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction conditions of Schisandra sphenanthera(SS)seed oil by supercritical CO2ex?traction and identify its components by GC-MS.Methods SS seed oil was used as tested material,response surface methodology was used to optimize the process of supercritical CO2extraction,and GS-MS method was used to analyse SS seed oil composition.Results A model of an equation was established,which could be used to optimize the process parameters of supercritical CO2extraction of SS seed oil. The optimum extraction parameters were as follows:the extraction pressure was 33 MPa,the extraction temperature was 53℃,the extraction time was 90 min,the CO2flow rate was 21.40 ml/min.In this condition,the extraction rate of SS seed oil was 7.97%.The SS seed oil was analyzed by GC-MS,and 23 compounds were identified.In these compounds,(1α,4a.β,8a.α)-1,2,3, 4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethenyl)-naphthalene,(-)-1,7-dimethyl-7-(4-methyl-3-pentenyl)-tricyc?lo[2.2.1.0(2,6)]heptane and(R)-2,4a,5,6,7,8-octahydro-3,5,5,9-tetramethyl-1H-(σ-phenyl)cycloheptene had the content of more than 10% and the contents were 27.78%,14.77% and 13.12% respectively.Conclusion This process has high extraction rate, fast speed and simple operation,and can be used for the extraction of SS seed oil.
