Effect of paeoniflorin on irritable bowel syndrome and barrier function of intestinal epithelia cell
10.13220/j.cnki.jipr.2017.10.010
- VernacularTitle:芍药苷对肠易激综合征的作用及肠上皮细胞屏障功能的影响
- Author:
Xiang-Hong LI
1
,
2
;
Ping-Xiang XU
;
Bei-Kang GE
;
Jia-Weng YANG
;
Yi MA
;
Wei-Jie YAN
;
Hui TIAN
;
Ming XUE
Author Information
1. 100069北京,首都医科大学基础医学院药理学系
2. 100069北京,北京市铁营医院药剂科
- Keywords:
irritable bowel syndrome(IBS);
paeoniflorin;
Caco-2 cell;
inflammation;
cell barrier function
- From:
Journal of International Pharmaceutical Research
2017;44(10):958-964
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the effect of paeoniflorin(PF)in ameliorating the irritable bowel syndrome(IBS)such as diarrhea and bellyache,and the barrier function of PF on intestinal epithelial cell and inflammation.Methods The diarrhea model was conducted by exposing rat to restraint stress stimulation and bellyache model was conducted by subcutaneous injection of neostig?mine to mice.The Caco-2 monolayer cell model with barrier dysfunction was established by trypsin stimulation and the inflammatory Caco-2 cell model was established by interleukin-1β(IL-1β)stimulation.On the basis of these models,effects of PF at different doses (low 14 mg/kg·d,medium 28 mg/kg·d,and high 56 mg/kg·d)on IBS syndromes and Caco-2 cell function were investigated. Re-sults PF could significantly reduce the frequency of defecation in diarrhea rat model(P<0.05)and relieve abnormal bowel move?ments in bellyache mice model(P<0.05).PF significantly increased TEER value(P<0.01),decreased the transmittance of fluores?cein(P<0.01)and up-regulated the expression of tight junction(ZO-1)protein(P<0.01).The gene and protein expression of nucle?ar factor profilin kappa Bα(IκBα)in inflammatory Caco-2 cell model was significantly improved(P<0.01)when treated with PF. Conclusion Our study proves for the first time that PF significantly ameliorated the diarrhea and bellyache symptoms of IBS in the di?arrhea model and bellyache model.The PF intervention effect on ZO-1 and IκBα protein might be one of the molecular mechanism of ameliorating the symptoms of IBS.
