Effect of Pirin on the proliferation and self-renew of glioma stem cell
10.13220/j.cnki.jipr.2017.10.009
- VernacularTitle:Pirin对脑胶质瘤干细胞增殖和自我更新的作用
- Author:
Hai-Lu JIANG
1
;
Feng LIU
;
Wen-Hui DONG
;
Jin WU
;
Jiang-Hong MAN
;
Xiao-Tao DUAN
Author Information
1. 军事医学科学院毒物药物研究所
- Keywords:
Pirin;
glioma stem cell(GSC);
cell ability;
tumor sphere
- From:
Journal of International Pharmaceutical Research
2017;44(10):953-957,969
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Pirin(an iron-binding nuclear protein)on the proliferation and self-renewal of glioma stem cell(GSC)so as to provide a potential therapy target for malignant glioma.Methods PLKO.1-shPirin lentiviral plasmids were constructed to stably knock down Pirin in GSC by using lentivirus infection system.The interference efficiency of Pirin short hair?pin RNA(shRNA)was detected by Western blotting. The capacity of GSC was examined by the assessment of cell viability. Tumor sphere formation assay was used to detect the effect of Pirin on GSC self-renewal capacity. Results Pirin was highly expressed in GSC.Consistently,the protein level of Pirin in the conditioned medium from GSC was much higher than that from the corresponding non-stem tumor cell(NSTC).Gene-sequencing analysis demonstrated that PLKO.1-shPirin lentiviral plasmids were successfully con?structed.Pirin shRNA transfection significantly inhibited the expression of Pirin in GSC and suppressed the cell viability and ability of tumorsphere formation.Conclusion Knocking down Pirin significantly inhibites the cell proliferation and self-renewal of GSC.
