Exploration on extraction and enrichment process of anti-inflammatoryand analgesic active-fraction(ARF)which containsmethyl salicylate glycosides in Dianbaizhu
10.13220/j.cnki.jipr.2017.09.011
- VernacularTitle:一种滇白珠富含水杨酸甲酯糖苷抗炎镇痛活性部位提取富集方法的探索
- Author:
Yi-Cheng ZHAO
1
;
Ting HE
;
Zhi-Ying WENG
;
Xiao-Yi CHEN
;
Shao-Juan BAI
;
Yan-Li CHANG
;
Yan WANG
;
Zi-Zhen LIU
;
Gai-Mei SHE
Author Information
1. 北京中医药大学中药学院
- Keywords:
Dianbaizhu;
anti-inflammatory and analgesic active-fraction;
HPLC;
extraction and enrichment;
fingerprint
- From:
Journal of International Pharmaceutical Research
2017;44(9):884-889
- CountryChina
- Language:Chinese
-
Abstract:
Objective The active ingredient was used as index to optimize the extraction and enrichment process of anti-in-flammatory and analgesic active-fraction(ARF)of Dianbaizhu. Methods Methyl salicylate triglycoside-B was chosen as index com-ponent to extract and enrich methyl salicylate glycosides. Extraction and elution solvents were optimized. The HPLC fingerprint was ob-tained with Thermo Hypersil Gold C18(250 mm×4.6 mm,5μm)column and a gradient elution with the mobile phase consisting of ace-tonitrile(A)-0.2％acetic acid(B)at a flow rate of 1.0 ml/min. And the detection wavelength was set at 294 nm. Results The opti-mized extraction solvent of Dianbaizhu was the 30％ethanol and the optimized elution solvent of ARF enriched by AB-8 macroporous resins was the 35％ethanol. The methodological study on similarity and RSD in ARF HPLC fingerprint of three batches of samples cor-responded to related regulations. Conclusion The extraction and enrichment process of ARF is stable and repeatable.
