Effect of umbilical cord mesenchymal stem cells on VEGF and MCP-1 of acute myocardial ischemia-reperfusion injury in rats
10.3760/cma.j.issn.1673-4181.2017.06.010
- VernacularTitle:脐带间充质干细胞移植对急性心肌缺血-再灌注损伤大鼠VEGF及MCP-1的影响
- Author:
Shijin CHEN
1
;
Yufang SHI
;
Bo ZHANG
;
Jun LIU
;
Song HAN
;
Wei LI
;
Huajiang DONG
;
Chongzhi SHANG
;
Huipeng MENG
;
Hongjun DING
;
Mingliang ZHAO
Author Information
1. 443000,宜昌市第二人民医院心血管内科
- Keywords:
Human umbilical cord mesenchymal stem cells;
Acute myocardial ischemia-reperfusion injury;
Vascular endothelial growth factor;
Monocyte chemoattractant protein-1
- From:
International Journal of Biomedical Engineering
2017;40(6):453-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human umbilical cord mesenchymal stem cells (UC-MSCs ) on vascular endothelial growth factor ( VEGF ) and monocyte chemoattractant protein-1 ( MCP-1 ) of acute myocardial ischemia-reperfusion (AMI-R) injury in rats. Methods 24 Sprague-Dawley rats were randomly divided into sham group, AMI-R group and UCMSCs treatment groups on average. The rats were sacrificed on the 10th day after UCMSCs transplantation, and the myocardial tissues below the ligature were taken. The mRNA and protein expressions of MCP-1 of the tissue were detected by RT-PCR and Western Blot respectively, and the expression of VEGF protein was detected by immunohistochemistry. Results The relative expression levels of MCP-1 mRNA and the protein in UCMSCs group were significantly lower than those in sham group and AMI-R group (all P<0.05). The expression of VEGF protein in UCMSCs group was significantly higher than that in sham group and AMI-R group, the differences were statistically significant(all P<0.05). Conclusion UCMSCs transplantation can promote the angiogenesis and decrease the inflammation reaction in the treatment of acute myocardial ischemia-reperfusion injury.
