Dynamic changes of ATP binding cassette transporter A1 expression in perihematomal tissue of mouse cerebral hemorrhage model induced by collagenase
10.3760/cma.j.issn.1673-4165.2018.09.008
- VernacularTitle:胶原酶诱导脑出血模型小鼠血肿周围组织ATP结合盒转运体A1表达的动态变化
- Author:
Xiangming TANG
1
;
Zixuan CHEN
;
Yingge WANG
;
Hongmei CHEN
;
Jiang XU
;
Zuowei DUAN
;
Xiaohong WANG
;
Jingyan LIANG
;
Xinjiang ZHANG
Author Information
1. 225000,扬州大学医学院
- Keywords:
Cerebral Hemorrhage;
ATP Binding Cassette Transporter 1;
Disease Models,Animal;
Mice
- From:
International Journal of Cerebrovascular Diseases
2018;26(9):689-695
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ATP binding cassette transporter A1 (ABCA1) expression in perihematomal tissue of mouse cerebral hemorrhage model induced by collagenase. Methods Stereotactic injection of type Ⅳ collagenase was used to induce a model of caudate putamen intracerebral hemorrhage in mice. The behavioral scores were use to assess neurological deficits at 24 h, 48 h and 72 h after model making. Neisserian staining was used to detect the morphology of neurons around hematomas.Immunohistochemical staining and Western blot analysis were used to detect the expression of ABCA1 around hematomas. Results Nissl bodies reduction, atrophy, necrosis of perihematomal neurons were observed and aggravated over time. Immunohistochemical staining and Western blot analysis showed that the expression level of ABCA1 in the perihematomal tissue was significantly higher than that in the sham operation group (all P < 0. 05), and the expression level increased significantly with time (all P < 0. 05 ).Conclusion ABCA1 was up-regulated after cerebral hemorrhage, suggesting that it might be involved in the pathological process of cerebral hemorrhage.
