Mechanisms of Naoshuantong capsule in the prevention of cold wave-induced stroke in hypertensive rats: a differential proteomics study
10.3760/cma.j.issn.1673-4165.2018.07.002
- VernacularTitle:脑栓通预防寒潮促发高血压大鼠卒中的机制:差异蛋白质组学研究
- Author:
Xiangpen LI
1
;
Xiaoni ZHANG
;
Lihuan LAN
;
Mei LI
;
Ruxun HUANG
;
Ying PENG
Author Information
1. 中山大学孙逸仙纪念医院神经科
- Keywords:
Cold Temperature;
Stroke;
Brain Ischemia;
Cerebral Hemorrhage;
Hypertension,Renal;
Proteomics;
Rats;
Naoshuantong Capsule
- From:
International Journal of Cerebrovascular Diseases
2018;26(7):484-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effect and mechanisms of Naoshuantong capsule in stroke induced by artificial cold wave in hypertensive rats.Methods A total of 130 SD rats were randomly divided into a sham operation group (n =30),a model control group (n =50) and a Naoshuantong treatment group (n=50;intragastric administration of Naoshuantong,0.5 g/kg,2/d for 7 days).Renovascular hypertensive rats model were established by two-kidney,two clip method.At 13th week after operation,rats were exposed to artificial cold wave for 3 days (12 h light of 22 ℃ and 12 h dark of 4 ℃,3 cycles).The brain tissue samples were extracted at the end of the experiment.Differential protein proteomic techniques were used for the identification,functional classification and preliminary analysis of the differentially expressed protein spots,and Western blot was used for the validation of some key proteins.Results There was no occurrence of stroke in the sham operation group,and the incidence of stroke in the model control group (36.00％,18/50) was significantly higher than that in the Naoshuantong treatment group (18.00％,9 / 50;x2 =4.110,P =0.043).With the two-dimensional gel electrophoresis analysis,6 different proteins were identified from 14 protein spots.Among them,the up-regulated superoxide dismutase 2 (SOD2) and the down-regulated B-cell lymphoma 10 (Bcl-10) were found to be at the central location of protein interactions,which has been verified by Western blot.Conclusion Naoshuantong can reduce the occurrence of stroke induced by artificial cold wave in renovascular hypertensive rats.SOD2 up-regulating and Bcl-10 downregulating may be involved in the mechanisms of of Naoshuantong in the prevention of cold wave-induced stroke in hypertensive rats.
