Caveolin-1 involvement of albumin in improving blood-brain barrier permeability after subarachnoid hemorrhage
10.3760/cma.j.issn.1673-4165.2018.03.007
- VernacularTitle:陷窝蛋白-1参与白蛋白改善蛛网膜下腔出血后血脑屏障通透性
- Author:
Lili XU
1
;
Hui CAO
;
Yao ZHANG
;
Yi XIE
;
Ruidong YE
;
Xinfeng LIU
Author Information
1. 210002,南京大学医学院附属金陵医院神经内科
- Keywords:
Albumins;
Caveolin-1;
Endothelium,Vascular;
Cells,Cultured;
Endocytosis;
Subarachnoid Hemorrhage;
Blood-Brain Barrier
- From:
International Journal of Cerebrovascular Diseases
2018;26(3):195-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of human serum albumin (Alb) on the permeability of blood-brain barrier (BBB) after subarachnoid hemorrhage (SAH) and the pathways for Alb uptake in endothelial cells.Methods Mouse brain endothelial cells (bEnd.3) were cultured in the Transwell chamber was used to induce a BBB model.A SAH in vitro model was induced by adding 10 μmol/L oxyhemoglobin into the culture medium.The cells were divided into 3 groups:control group,SAH group,and Alb group (10 mg/ml).Transendothelial electric resistance (TEER) was used to detect the permeability of BBB.A confocal microscope was used to observe whether the fluorescent labeled Alb could be uptaken by bEnd.3cells.Immunoprecipitation was used to detect whether Alb could interact with the cells of caveolin 1 (Cav-1).According to the principle of siRNA,Cav-1 siRNA was transfected into bEnd.3 cells to inhibit the expression of Cav-1.Western blot analysis was used to detect whether bEnd.3 cells could uptake Alb.TEER was used to detect the permeability of BBB.Results Compared with the SAH group,the TEER value of the Alb group increased significantly (P =0.011).Alb was uptaken by bEnd.3 cells and interacted with Cav-1 in bEnd.3 cells.Cav-1 siRNA transfection could significantly inhibit the expression of Cav-1 in bEnd.3cells and reduce the uptake ability of Alb by cells (P=0.025),resulting in a significant decrease in the protective effect of Alb on BBB (P ＜ 0.001).Conclusion Cav-1 may be uptaken by endothelial cells under the participation of Cav-1 and improve the permeability of BBB after SAH.
