Clinical efficiency analysis of Mycobacterium tuberculosis detected by LAMP technique
10.3969/j.issn.1673-4130.2018.13.023
- VernacularTitle:LAMP技术检测结核分枝杆菌的临床时效性分析
- Author:
Yi CHEN
1
;
Wenyan HUANG
;
Junli DENG
Author Information
1. 深圳市宝安区慢性病防治院检验科
- Keywords:
Mycobacterium tuberculosis;
loop-mediated isothermal amplification;
tuberculosis;
diagnosis
- From:
International Journal of Laboratory Medicine
2018;39(13):1622-1624,1628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical value of a loop-mediated isothermal amplification (LAMP) method for detection of Mycobacterium tuberculosis in the application of primary medical institutions . Methods The sputum samples from 140 patients with clinically diagnosed pulmonary tuberculosis detected by LAMP and anti-acid staining method .McNemar test and Kappa test were used for statistical analysis . Results In 140 cases of sputum ,the positive detection rate of anti-acid staining and LAMP method was 29 .3%(41/140)and 45 .0%(63/140) .Compared with the smear method ,the sensitivity of LAMP method was 97 .6%(40/41) ,the specificity was 76 .8%(76/99) .The overall consensus rate was 82 .9%(116/140) .Com-pared with the two methods ,the consistency between the two is moderate and statistically significant (Kappa＝0 .642 ,P＜0 .05) .Compared with the smear method ,the LAMP method used in large-scale specimen examination takes shorter time .Conclusion In sputum sample detection ,positive detection rate of LAMP method is better than antacid staining smear method .It has good batch detection ability and good clinical application prospect .
