Effect analysis of improving the HIV positive detection rate by single sample nucleic acid amplification test in Shenzhen
10.3969/j.issn.1673-4130.2018.13.008
- VernacularTitle:运用单样本核酸扩增检测技术对提高深圳地区HIV检出率的效果分析
- Author:
Songxing WANG
1
;
Wen XIONG
;
Xinghui GU
;
Heng LIU
;
Yunping XU
;
Jinfeng ZENG
Author Information
1. 深圳市血液中心
- Keywords:
nucleic acid amplification test;
enzyme-linked immuno sorbent assay;
human immunodefi-ciency virus;
window period;
blood safety
- From:
International Journal of Laboratory Medicine
2018;39(13):1562-1565
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of improving the human immunodeficiency virus (HIV)posi-tive detection rate by single sample nucleic acid amplification test (SS-NAT) in Shenzhen ,and to explore the effect of SS-NAT on reducing the risk of HIV infection in transfusion .Methods 269 228 blood samples were performed parallel detection by SS-NAT (Procleix Tigris ) and two kinds of enzyme-linked immuno sorbent assay(ELISA)reagents ,and then the samples with nonreactive by ELISA and reactive by SS-NAT were tested by HIV identification assay .The blood donors with reactive HIV identification assay were made tracing tests . All the samples with reactive by ELISA or HIV identification assay were sent to the Shenzhen Center for Dis-ease Control and Prevention (CDC) for Western Blot (WB) diagnostic tests .Results The samples with reac-tive by the third generation ELISA reagents ,the fourth generation ELISA reagents ,both ELISA reagents and SS-NAT were 188 ,340 ,422 and 103 ,which reactive rate was 0 .698‰(188/269 228) ,1 .263‰(340/269 228) , 1 .567‰(422/269 228) and 0 .383‰(103/269 228) ,respectively .We found four samples with nonreactive by ELISA but reactive by SS-NAT .The four donors were found HIV reactive by both ELISA and SS-NAT after tracing .All the samples with reactive by ELISA or HIV identification assay were sent to CDC for confirmatory tests and 103 of them were positive .The positive detection rate of transfusion-transmissible HIV infection af-ter ELISA detection was 1∶67 307(4/269 228) .Conclusion The application of SS-NAT in blood screening can improve the HIV positive detection rate ,shorten window period of HIV detection and reduce residual risk of transfusion-transmissible HIV infection ,and then blood safety can be effectively improved .
