The detection of HLA-B27 gene and gene typing of ankylosing spondylitis patients by fluorescence PCR
10.3969/j.issn.1673-4130.2018.13.005
- VernacularTitle:荧光定量PCR染料法检测强直性脊柱炎患者HLA-B27基因及分型
- Author:
Xiuxia LUO
1
;
Zhihua YIN
;
Chunrong ZHANG
;
Jie CHEN
;
Zhizhong YE
;
Jinxian HUANG
Author Information
1. 深圳市福田区风湿病专科医院
- Keywords:
human leucocyte antigen-B27;
fluorescence polymerase chain reaction;
flow cytometry;
ankylosing spondylitis
- From:
International Journal of Laboratory Medicine
2018;39(13):1550-1552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical application value of fluorescence polymerase chain reaction (PCR) .in the human leucocyte antigen-B27(HLA-B27) gene and gene typing detection of ankylosing spondy-litis (AS) patients .Methods A total of 43 clinical blood samples of AS and 56 samples of healthy controls were collected in Shenzhen Futian hospital for rheumatic diseases from January 2014 to March 2015 .HLA-B27 gene was detected by flow cytometry .HLA-B27 gene and gene typing was also detected by the fluorescence PCR method .Results Among 43 samples ,40 samples were HLA-B27 positive(93 .02%) by flow cytometry while 39 samples were HLA-B27 positive (90 .70%) by fluorescence PCR .The total coincidence rate was 97 .50% .Among 39 positive samples ,32 samples were HLA-B2704 positive (82 .05%) and 7 samples were HLA-B2705 positive (17 .95%) .Conclusion The fluorescence PCR is an accurate method to detect HLA-B27 gene and presents high consistency with flow cytometry .It can also detect the HLA-B27 gene typing .It may have great clinical application value and prospects .
