Analysis of CYP2D6 gene polymorphism of Chinese population in Hubei
10.3969/j.issn.1673-4130.2018.13.002
- VernacularTitle:湖北人群CYP2D6多态性的初步研究
- Author:
Rong ZENG
1
;
Xiaobing MAO
;
Xiang LIU
;
Jing HUANG
Author Information
1. 湖北中医药大学检验学院
- Keywords:
CYP2D6 gene;
polymorphism;
polymerase chain reaction-restriction fragment length poly-morphism
- From:
International Journal of Laboratory Medicine
2018;39(13):1540-1542,1546
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminary study the distribution of CYP2D6 polymorphism in Hubei popula-tion ,with the intention of solid base for further applied research .Methods Venous blood was collected from 137 volunteers ,analysed CYP2D6 * 10 Gene Polymorphism by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method .genotypes can be distinguished by agarose gel electro-phoresis and gene sequencing .Hardy-Weinberg equilibrium law could be used to detect whether the genotype distribution was balanced ,and chi-square test was used for verification ,and the results were compared with the previous literature .Results Among the 137 samples ,wild type (CC) was 35 ,the frequency was 25 .5% .Het-erozygote (CT) was 52 ,the frequency was 38 .0% .And mutant (TT) was 50 ,the frequency was 36 .5% .Also , Callele frequency was 44 .5% while the Tallele was 55 .5% .There was no statistical difference compared with previous studies (P＞0 .05) .Conclusion Since there is a high mutation frequency of CYP2D6*10 in Hubei population ,it is very necessary to carry out genotyping to guide clinical medication correctly .
