High resolution melting analysis for detection of ALDH 2 and ADH1B gene polymorphisms
10.3969/j.issn.1673-4130.2018.13.001
- VernacularTitle:高分辨熔解曲线分析技术检测ALDH2和ADH1B基因多态性
- Author:
Shupeng JIANG
1
;
Yongqing TONG
;
Rui ZHAO
;
Yan LI
Author Information
1. 武汉大学人民医院检验科
- Keywords:
high-resolution melting;
alcohol dehydrogenase-1B;
aldehyde dehydrogenase 2;
single nucleotide polymorphism
- From:
International Journal of Laboratory Medicine
2018;39(13):1537-1539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the system of high resolution melting for detection of aldehyde dehydro-genase 2(ALDH2) and alcohol dehydrogenase-1B (ADH1B) gene polymorphisms .Methods The short prim-ers were designed for ALDH2 and ADH1B gene .Different PCR products were analyzed using Eva Green dyes after amplification ,which were confirmed by Sanger sequencing .Results The genotypes of ALDH2 rs671 and ADH1B rs1229984 were successfully detected in the same procedure by HRM within 90min ,and the results were consistent with Sanger sequencing .Conclusion The assay of HRM is simple ,rapid ,cost-effective ,and re-liable for the detection of ALDH2 and ADH1B polymorphism and it is worthy to be popularized .
