Nucleic acid dye GeneGreen can influence the quality of DNA bands in agarose gel electrophoresis
10.3969/j.issn.1673-4130.2018.11.002
- VernacularTitle:核酸染料GeneGreen可影响DNA琼脂糖凝胶电泳条带的质量
- Author:
Qiang MA
1
;
Yan CAI
;
Lei XU
;
Hebin LIAO
;
Jiang ZOU
;
Ru SUN
;
Xiaolan GUO
Author Information
1. 川北医学院附属医院检验科
- Keywords:
gel electrophoresis;
ethidium bromide;
DNA bands
- From:
International Journal of Laboratory Medicine
2018;39(11):1286-1288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the causes of twisted or tailed DNA bands in agarose gels after electro-phoresis .Methods Prestained and poststained methods were employed to exam 3 kinds of DNA marker bands in agarose gel with GeneGreen and Ethidium Bromide ,respectively .Results Three kinds of DNA marker bands in agarose gel with 1:10000 and 1:5000 concentration of GeneGreen showed obvious distortions and trailing phenomena compared with the same concentration of Ethidium Bromide w hen the prestained method was used for electrophoresis ,which were improved when the poststained method was used in agarose gel with GeneGreen and Ethidium Bromide ,respectively .Conclusion Nucleic acid dye GeneGreen can affect the quali-ty of DNA bands in agarose gel electrophoresis .When the quality of DNA itself ,agarose gel quality ,electro-phoretic fluid quality and voltage are excluded ,the quality of nucleic acid dye should be taken into considera-tion if the bands twisting or tailing occurs when the DNA nucleic acid electrophoresis is carried out by pres-tained method .The quality of DNA electrophoresis bands can be improved by using poststained method or re-placing nucleic acid dye .
