The effect of membrane protein NMHC ⅡA on invasion and metastasis of bladder cancer cells
10.3969/j.issn.1673-4130.2018.11.001
- VernacularTitle:膜蛋白NMHCⅡA的表达对膀胱癌细胞侵袭和转移的影响
- Author:
Dan XIONG
1
;
Xiaoqing XU
;
Lijuan KAN
;
Huamei TANG
;
Yumin LI
;
Shuilan ZHANG
;
Fangyong LI
;
Hongmei MO
;
Xiuming ZHANG
Author Information
1. 深圳大学第三附属医院医学检验科
- Keywords:
non-muscle myosin heavy chain ⅡA;
bladder cancer;
invasion;
metastasis
- From:
International Journal of Laboratory Medicine
2018;39(11):1281-1285
- CountryChina
- Language:Chinese
-
Abstract:
Objective Non-muscle myosin heavy chain ⅡA (NMHC ⅡA ) plays a significant role in tumor progression and metastasis .Our prior study showed that the expression of NMHC ⅡA was much higher in human bladder cancer sample than that in adjacent tissue .The increased level of NM HC ⅡA expression was correlated with worse prognosis .However ,the role of NMHC ⅡA is unknown in the invasion and metastasis of bladder cancer .Methods RT-PCR and western blotting were used to examine NMHC ⅡA expression lev-els in normal bladder epithelial cells and bladder cancer cell lines .T he migration and invasion ability of cells was tested by wound healing assay and Transwell invasion assay ,respectively .Results Our study showed that knockdown of NMHC ⅡA inhibited migration and invasion in bladder cancer cell line .Conclusion The study indicated that NM HC ⅡA expression increased the invasion and metastasis ability of bladder cancer cell line in vitro .
